VitroView™ Gram Stain Kit for Slide Smear (30 ml per Dropper bottle) SKU: VB-2000s $35. VitroView™ Twort’s Counterstain Kit SKU: VB-2001 $159.00 User Manual and Material Safety Data Sheet (MSDS) (PDF) Avoid contact with eyes, skin and clothing. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. This product is intended for research purposes only. Observe the results of the staining procedure under oil immersion using a bright field microscope.Alternatively, the slide may be shaken to remove most of the water and air-dried. Blot with bibulous paper to remove the excess water. Wash off the red safranin solution with water.Flood slide with counterstain, Gram’s safranin Solution.Further delay will cause excess decolorization in the gram-positive cells, and the purpose of staining will be defeated. Decolorize with Gram’s Decolorizer Solution until the blue dye no longer flows from the smear.Shake off the excess water from the surface. Pour off the iodine solution and gently wash with tape water.Allow it to remain for Gram’s Crystal Violet Solution. Wash slide in a gentle and indirect stream of tap water for 2 seconds.Please note that the quality of the smear (too heavy or too light cell concentration) will affect the Gram Stain results. Gram, Gram positive & Gram negative bacteria, artificial control slides contain a section of gram positive and gram negative staining rat lung and a section. Flood fixed smear of cells with Gram’s Crystal Violet solution.The applied heat helps the cell adhesion on the glass slide to make possible the subsequent rinsing of the smear with water without a Allow to air dry and fix it over a gentle flame, while moving the slide in a circular fashion to avoid localized overheating. It is possible to put 3 to 4 small smears on a slide, if more than one culture is to be examined.Spread the culture with an inoculation loop to an even thin film over a circle of 1.5 cm in diameter.A visual detection of the culture on an inoculation loop already indicates that too much is taken. It should only be a very small amount of culture. If the culture is to be taken from a Petri dish or a slant culture tube, first add a drop or a few loopful of water on the slide and aseptically transfer a bit of the colony. Transfer a drop of the suspended culture to be examined on a slide with an inoculation loop.Use a glass etching tool to mark one or more dime-sized circles on the surface of a slide.This kit is designed for the gram staining of slide smear sample. Gram-negative cells have a thin peptidoglycan layer and stain red to pink. Gram-positive cells have a thick peptidoglycan layer and stain blue to purple. The staining procedure differentiates organisms of the domain Bacteria according to cell wall structure. The Gram stain is fundamental to the phenotypic characterization of bacteria.
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